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采用两种识别不同表位的小鼠抗人白细胞介素2受体(IL-2R)单克隆抗体(McAb),在国内首次建立了检测可溶性IL-2R(sIL-2R)的夹心法ELISA,并进行了初步应用。结果显示:本法特异性强、敏感性高、重复性好、操作简便,能检出正常人、某些患者血清中以及细胞培养上清中sIL-2R,可用于基础和临床免疫学研究。
Sandwich ELISA for detecting soluble IL-2R (sIL-2R) was established for the first time in China using two monoclonal antibodies against human interleukin 2 receptor (IL-2R) that recognize different epitopes. And carried out a preliminary application. The results show that this method is specific, sensitive, reproducible and easy to operate. It can detect sIL-2R in serum of patients and some patients, and can be used in basic and clinical immunology research.