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目的研究环氧合酶抑制剂NS398对唾液腺腺样囊性癌(SACC)-83细胞的抑制作用。方法2008年6—12月于中国医科大学中心实验室,用含0、50、100、150、200μmol/LNS398的培养液体外培养SACC-83细胞24、48、72、96h后,应用噻唑蓝(MTT)快速比色法,检测NS398对SACC-83细胞的抑制作用;用上述实验确定的最大抑制浓度培养液培养细胞,采用倒置相差显微镜和透射电镜观察细胞形态。结果随着作用时间延长和药物浓度增加,NS398对SACC-83细胞的抑制率逐渐增加。NS398在浓度150μmol/L作用72h后,抑制作用最明显。倒置相差显微镜观察,随着培养时间的增加,试验组(150μmol/LNS398)贴壁细胞数量明显减少,胞浆开始脱水产生空泡,并与胞膜融合,出现胞膜空泡化,胞核深染,胞质浓缩;透射电镜观察,试验组(150μmol/LNS398)细胞出现凋亡现象。结论NS398可抑制SACC-83细胞的增殖,并具有时间和浓度依赖性,NS398可能在唾液腺腺样囊性癌治疗中发挥重要作用。
Objective To study the inhibitory effect of cyclooxygenase inhibitor NS398 on salivary adenoid cystic carcinoma (SACC) -83 cells. METHODS: SACC-83 cells were cultured in culture medium containing 0, 50, 100, 150 and 200 μmol / L NS398 for 24, 48, 72 and 96 hours in the Central Laboratory of China Medical University from June to December in 2008, MTT) assay was used to detect the inhibitory effect of NS398 on SACC-83 cells. The cells were cultured in the medium with the maximum inhibitory concentration determined by the above experiment. The morphology of cells was observed by inverted phase contrast microscope and transmission electron microscope. Results With the prolongation of action time and the increase of drug concentration, the inhibitory rate of NS398 on SACC-83 cells gradually increased. NS398 in the concentration of 150μmol / L effect 72h, the most obvious inhibition. The inverted phase contrast microscope showed that the number of adherent cells in the test group (150μmol / L NS398) decreased significantly as the culture time increased, and the cytoplasm began to dewate to produce vacuoles, which fused with the membrane, with membrane vacuolization and deep nucleus Staining and cytoplasm concentration. Transmission electron microscopy showed apoptosis in the test group (150μmol / L NS398). Conclusion NS398 can inhibit the proliferation of SACC-83 cells in a time and concentration-dependent manner. NS398 may play an important role in the treatment of salivary adenoid cystic carcinoma.