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用人工精浆将虹鳟精液稀释100倍,然后置于家用电冰箱内,在4℃的情况下,可保存30天乃至50天以上。用此保存精液进行人工授精,可获得良好效果。用保存30天的精子授精,发眼率和孵化率分别可达53%和52%以上。另外,此方法还可活化虹鳟精巢内的精子,便于人工授精。这种低温保存法简单、实用,容易推广,对鱼类的苗种生产和遗传育种工作具有重要意义。文中就人工精浆的制备、精液的保存方法及保存精液的运动性和授精效果等问题作了详细论述。
Using artificial semen to dilute Rainbow trout semen 100 times, and then placed in a household refrigerator at 4 ℃, can be stored for 30 days or even more than 50 days. Use this to save the sperm artificial insemination, get good results. With 30 days of sperm preservation, eye rate and hatching rates were 53% and 52% respectively. In addition, this method can also activate sperms in rainbow trout testis to facilitate artificial insemination. This method of cryogenic preservation is simple, practical and easy to popularize. It is of great significance to the fish seed production and genetic breeding. In this paper, the preparation of artificial semen, preservation of semen and preservation of semen motility and insemination effects and other issues were discussed in detail.