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目的探讨华蟾素对卵巢3AO细胞基质金属蛋白酶(MMP-2)和组织抑制因子(TIMP-2)表达的影响及其临床意义。方法体外培养卵巢癌3AO细胞,不同浓度的华蟾素干预后,用SABC免疫组化法检测MMP-2和TIMP-2表达。结果卵巢癌3AO细胞可表达MMP-2和TIMP;0.25mg/ml华蟾素对卵巢3AO细胞MMP-2和TIMP-2的表达无显著影响(P>0.05);2.5mg/ml华蟾素干预后,卵巢癌3AO细胞MMP-2的表达显著下降,TIMP-2的表达显著升高(P<0.05);25mg/ml与2.5mg/ml华蟾素对卵巢癌3AO细胞MMP-2和TIMP-2表达影响无统计学差异(P>0.05)。结论 2.5mg/ml华蟾素可显著降调卵巢癌3AO细胞MMP-2的表达,升调TIMP-2的表达,两者的失衡可抑制卵巢癌3AO细胞的侵袭性生长。
Objective To investigate the effect of cinobufacini on the expression of matrix metalloproteinase (MMP-2) and tissue inhibitor of metalloproteinase (TIMP-2) in ovarian 3AO cells and its clinical significance. Methods Ovarian cancer 3AO cells were cultured in vitro. SABC immunohistochemistry was used to detect the expression of MMP-2 and TIMP-2 after intervention with different concentrations of cinobufacini. Results Overexpression of MMP-2 and TIMP in ovarian cancer 3AO cells did not affect the expression of MMP-2 and TIMP-2 in ovarian 3AO cells (P> 0.05) (P <0.05). At 25 mg / ml and 2.5 mg / ml of cinobufacini, the expression of MMP-2 and TIMP-2 in ovarian cancer 3AO cells were significantly decreased 2 expression no significant difference (P> 0.05). Conclusion 2.5mg / ml of cinobufacini can significantly down-regulate the expression of MMP-2 and up-regulate the expression of TIMP-2 in 3AO cells. The imbalance between the two can inhibit the invasive growth of 3AO cells.