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目的 研究辛伐他汀对原代培养的骨髓基质细胞骨形成蛋白 - 2 (BMP- 2 )表达及碱性磷酸酶活性的影响 ,探讨其刺激成骨的作用机制。 方法 体外培养成年小鼠骨髓基质细胞 ,以不同浓度 (0、0 .1、 0 .2、0 .5和 1 .0μmol/L )的辛伐他汀、基因重组人 BMP- 2 (1 0 0 ng/ml)作用 72小时碱性磷酸酶活性的酶学测定 ;免疫细胞化学染色 ,Western Blotting检测 BMP- 2表达的变化。 结果 辛伐他汀作用 72小时后 ,细胞中的 BMP- 2表达水平增高 ,随浓度增加 ,表达量增加 ,对照组少量表达 ;碱性磷酸酶活性显著增加 ,呈剂量依赖关系 ,辛伐他汀 0 .5和 1 .0 μmol/L与对照组间具有统计学意义 (P<0 .0 5)。 结论 辛伐他汀可促进骨髓基质细胞中 BMP- 2的高表达 ,细胞自分泌或旁分泌BMP- 2增多 ,碱性磷酸酶活性增高 ,有促进成骨的作用
Objective To study the effects of simvastatin on the expression of bone morphogenetic protein 2 (BMP-2) and alkaline phosphatase (AKP) in primary cultured bone marrow stromal cells (BMSCs) and explore the mechanism of simvastatin. Methods Adult mouse bone marrow stromal cells were cultured in vitro. Simvastatin with different concentrations (0, 0.1, 0.2, 0.5 and 1.0 μmol / L), recombinant human BMP-2 / ml) for 72 hours alkaline phosphatase activity; Immunocytochemical staining, Western Blotting detection of BMP-2 expression changes. Results After simvastatin treatment for 72 hours, the expression of BMP-2 in the cells increased. With the increasing of concentration, the expression of BMP-2 increased and the expression of BMP-2 increased slightly in the control group. The activity of alkaline phosphatase increased significantly in a dose-dependent manner. 5 and 1. 0 μmol / L and control group was statistically significant (P <0. 05). Conclusion Simvastatin can promote the high expression of BMP-2 in bone marrow stromal cells, increase autocrine or paracrine BMP-2, increase alkaline phosphatase activity and promote osteogenesis