论文部分内容阅读
目的探讨前β细胞克隆增强因子(PBEF)、血管内皮生长因子(VEGF)和肌球蛋白轻链磷酸化(pMLC)在人脐静脉内皮细胞(HUVEC)缺氧复氧中的表达及相互间的相关性。方法体外常规培养HUVEC细胞,实验分为对照组、缺氧组和缺氧复氧组三组,每组10个培养板孔细胞；Western blot ing检测三组PBEF、VEGF和pMLC蛋白表达；并分析PBEF、VEGF和pMLC三组在HUVEC缺氧复氧中的相关性。结果 HUVEC细胞缺氧后,PBEF,VEGF和pMLC蛋白表达较对照组均显著升高(<0.01)；缺氧组HUVEC细胞复氧12h后,PBEF,VEGF和pMLC蛋白表达较单纯缺氧组进一步升高,比较差异有统计学意义(<0.01)。 PBEF、VEGF和pMLC蛋白在HUVEC缺氧复氧中的表达均呈正相关；PBEF和VEGF间的等级相关系数为0.493,PBEF和pMLC间的等级相关系数为0.534,VEGF和pMLC间的等级相关系数为0.469。结论 PBEF、VEGF和pMLC参与HUVEC缺氧复氧过程,且三者在HUVEC缺氧复氧中的表达之间均呈正相关。“,”Objective To discuss the expression and relationship of pre-βcel colony-enhancing factor(PBEF),vessel endothelial growth factor(VEGF)and phosphorylation of myosin light chains(pMLC)in anoxia-reoxygenation of human umbilical vein endothelial cel (HUVEC).Methods HUVEC cel s were cultivated by the conventional method in vitro and divided into control,anoxia and anoxia-reoxygenation groups with 10 hole cel culture plate each.Expression of PBEF,VEGF and pMLC protein in every group were detected by western blot ing and analyzed the cor elation in anoxia-reoxygenation of HUVEC.Results Expression of PBEF,VEGF and pMLC protein were obviously higher than control group after HUVEC cel s of oxygen ( <0.01).Expression of PBEF,VEGF and pMLC protein increased more in HUVEC cel s of anoxia group after reoxygenation 12 h than anoxia group with statistical y significances ( <0.01).There are positive cor elations among expression of PBEF, VEGF and pMLC protein in HUVEC anoxia-reoxygenation.Rank cor elation coef icient was 0.493 between PBEF and VEG.Rank cor elation coef icient was 0.534 between PBEF and pMLC.Rank cor elation coef icient was 0.469 between VEGF and pMLC.Conclusion PBEF,VEGF and pMLC participated HUVEC anoxia-reoxygenation process and had a significant cor elation in HUVEC anoxia-reoxygenation.