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利用基因枪法将含有３个不同基因（ｈｐｔ，ｇｕｓ和ｇｎａ）的质粒ｐＷＲＧ１５１５和ｐＲＳＳＧＮＡ１共同转化粳稻品种鄂晚５号成熟胚诱导的愈伤组织．共再生出３５株独立转基因植株．ＰＣＲ／Ｓｏｕｔｈｅｒｎ印迹法分析发现，８３ ％的转基因植株含有所有３个外源基因．Ｗｅｓｔｅｒｎ印迹法分析发现 ７９％的含 ｇｎａ基因的转基因植株以不同水平表达ＧＮＡ。遗传分析证实外源基因在转基因植株后代中以孟德尔方式遗传．从其Ｒ１代亲本为孟德尔３：１方式遗传的Ｒ２代中，鉴定出２个含有所有３个外源基因的独立转基因植株纯系．这些纯系具有相似的外源基因表达量．褐飞虱喂养试验表明，这些纯系对褐飞虱具有显著的抑制作用．这些揭飞虱抗性提高的转基因纯系将应用于水稻抗虫育种中．实验证明，通过遗传转化和筛选可获得含在农业上有应用价值基因的转基因水稻纯系．
Plasmids pWRG1515 and pRSSGNA1, containing three different genes (hpt, gus and gna), were co-transformed into callus induced by mature embryos of Japonica rice E No.5 using a gene gun method. A total of 35 independent transgenic plants were regenerated. PCR / Southern blot analysis revealed that 83% of the transgenic plants contained all three foreign genes. Western blot analysis found that 79% of gna gene-containing transgenic plants expressed GNA at different levels. Genetic analysis confirmed that exogenous genes are mendelian in the offspring of transgenic plants. In R2 generation, which was inherited from Mendel’s 3: 1 pollen via R1 generation, two independent transgenic lines with all three foreign genes were identified as pure lines. These lines have similar amounts of exogenous gene expression. Nilaparvata lugens feeding tests showed that these pure lines had a significant inhibitory effect on N. lugens. These transgenic lines with increased resistance to planthopper will be used in rice insect resistance breeding. Experiments show that transgenic rice lines with genetic value of agriculture can be obtained through genetic transformation and screening.