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目的建立同时测定大鼠血清中尿苷和表告依春浓度的LC-MS/MS法;以尿苷和表告依春为板蓝根颗粒的指标成分,利用建立的LC-MS/MS法研究大鼠服用板蓝根颗粒后的药物动力学特征。方法色谱柱为Agela C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(体积比为5∶95),利用沉淀蛋白法,以多反应监测(multiple reaction monitoring,MRM)扫描方式检测大鼠给予板蓝根颗粒后血清中尿苷和表告依春的血清浓度。结果血清中尿苷和表告依春质量浓度分别在40.0~10 000.0μg·L-1和4.0~1 000.0μg·L-1内呈良好的线性关系;日内与日间精密度均小于15%,回收率和基质效应均在85%~115%之间。大鼠灌胃给予板蓝根颗粒后,尿苷的ρmax增至本底水平的2倍左右,表告依春的ρmax、tmax和t1/2分别为(0.61±0.08)mg·L-1、(2.25±0.61)h和(3.64±1.47)h。结论该方法适用于大鼠血清中尿苷和表告依春的测定及药物动力学研究。
OBJECTIVE To establish a LC-MS / MS method for the simultaneous determination of uridine and epirubicin in rat serum by LC-MS / MS method. Pharmacokinetics of Banlangen granules in mice. Methods The chromatographic column was an Agela C18 column (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-water (volume ratio 5:95) Serum concentrations of uridine and epididymal eosin in rats given Banlangen granules were detected. Results Serum uridine and ephedrine according to the quality of the concentration of 40.0 ~ 10 000.0μg · L-1 and 4.0 ~ 1 000.0μg · L-1 showed a good linear relationship; intra-day and day precision were less than 15% , Recovery and matrix effects are between 85% and 115%. After intragastric administration of Banlangen granules, the ρmax of uridine increased to about 2 times the background level. The values of ρmax, tmax and t1 / 2 were (0.61 ± 0.08) mg · L-1 and (2.25 ± 0.61) h and (3.64 ± 1.47) h. Conclusion This method is suitable for the determination of uridine and epidotoxin in rat serum and pharmacokinetic study.