论文部分内容阅读
目的 构建带有HIV 1gag或 gp12 0基因的重组腺病毒伴随病毒AAV HIV gag或AAV HIVgp12 0。方法 共转染法获取重组AAV HIV ;免疫酶法检测病毒滴度。结果 测定制备的重组腺病毒伴随病毒AAVgag42 ,AAVgp42 ,AAVgpRj6 ,病毒滴度在 10 4～ 10 5 范围。结论 构建了带有 gag和gp12 0基因的重组AAV HIV ,可以感染真核细胞并有较高的表达 ,可以用于做靶细胞 ,为发展新型的HIV 1重组AAV病毒载体活疫苗打下基础
Objective To construct recombinant adenoviruses with HIV 1 gag or gp12 0 gene as either AAV HIV gag or AAV HIV gp12 0. Methods Recombinant AAV HIV was obtained by co-transfection method. The virus titer was detected by immunoenzymatic method. Results The recombinant adenoviruses with virus AAVgag42, AAVgp42 and AAVgpRj6 were prepared and the virus titers ranged from 10 4 to 10 5. Conclusion Recombinant AAV HIV with gag and gp12 0 genes can be infected and expressed in eukaryotic cells and can be used as target cells to lay a foundation for the development of a new HIV A recombinant live vector vaccine