Role of CD80 in stimulating T lymphocyte activation

来源 :World Journal of Gastroenterology | 被引量 : 0次 | 上传用户:YouZiTou
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
AIM:To observe biological characteristics of hepatocar-cinoma cells before and after CD80 transfection and tocompare the effect of CD80-transfected hepatocarcinomacells on T lymphocyte activation.METHODS:Retro virus vector carrying CD80 genewas transfected into HepG2 cells to establish CD80-transfected hepatocarcinoma cells(HepG2/hCD80).Flowcytometry(FCM)was performed to detect CDS0 expres-sion in the transfected cells.RT-PCR was used to evalu-ate CD80 expression at mRNA level.In the presence ofanti-CD3 mAb,the proliferation of T lymphocyte wasobserved by MTT.Meanwhile,the expression of activatedmolecule marker CD25 was analyzed through FCM.RESULTS:A stable cell line HepG2/hCD80 expressingthe human CD80 was established.Growth curve showedthat the molecule CD80 could obviously decrease thegrowth of tumor cells.HepG2/hCD80 was evidenced tohave a potency to enhance T cell proliferation and up-regulate CD25 expression.CONCLUSION:CD80 transfection can lower ma-lignant phenotype of hepatocarcinoma cells.CD80transfection has a down-regulatory effect to activatedT cells in vitro. AIM: To observe biological characteristics of hepatocar-cinoma cells before and after CD80 transfection and tocompare the effect of CD80-transfected hepatocarcinoma cells on T lymphocyte activation. METHODS: Retro virus vector carrying CD80 gene was transfected into HepG2 cells to establish CD80-transfected hepatocarcinoma cells ( HepG2 / hCD80) .Flowcytometry (FCM) was performed to detect CDS0 expres-sion in the transfected cells. RT-PCR was used to evaluate-ate CD80 expression at mRNA level. The presence of anti-CD3 mAb, the proliferation of T lymphocyte wasobserved by MTT.Meanwhile, the expression of activated molecule marker CD25 was analyzed through FCM.RESULTS: A stable cell line HepG2 / hCD80 expressing the human CD80 was established. Growth curve showed that the molecule CD80 could significantly reduce the growth of tumor cells. HepG2 / hCD80 was evidenced tohave a potency to enhance T cell proliferation and up-regulate CD25 expression. CONCLUSION: CD80 transfection can lower ma-lignant phenotype of hepatocar CD80 transfection has a down-regulatory effect to activated T cells in vitro.
其他文献
Mucinous cystic neoplasms (MCNs) of the pancreas are a distinct entity, account for 1% of pancreatic exocrine tumors. MCNs can be classified histologically as a
目的:应用脉冲场凝胶电泳(PFGE)技术对不同来源的肠炎沙门菌进行分子分型,为政府部门修订相关的卫生法规提供了重要科学依据。方法:采用限制性内切酶Xba I,对广州地区2009-20
一、无机非金属新材料主持人:无机非金属新材料对普通老百姓来说,是个陌生的概念。但说起玻璃纤维和特种玻璃纤维、玻璃钢/复合材料、人工晶体、工业陶瓷等,大家就不陌生了
The sorption of AuCl_4~-, AuCl_2~- and Au(S_2O_3)_2~(3-) on δ-MnO_2 was investigated at pH2—11.6, 0.01 mol/L and 0.1 mol/L NaNO_3 solutions. At pH 4 in two e
王子钢公司是日本最大的扁钢生产厂,最近又有一个新的小型钢厂投产。新厂投资7900万美元,拥有一台80t节能电炉,采用排出的废气对废钢进行预加热。这台直流电弧炉由Daido钢公
上世纪五十年代中期,我的亲生母亲因患重病,无钱医治,英年早逝,年仅38岁。我14岁从南方来到天津上初中,与早就在天津工作的父亲一起生活。不久继母进入我的家庭,我的心里别提多难过了,一直不舒服,耿耿于怀。每每想起我的亲生母亲,呵护我、关爱我,泪水不断往外涌。于是,我就时不时地与继母闹别扭,有时候还发生激烈争吵,甚至离家出走。  我上初中时,有位很要好的同学,他也是从小没有了母亲,后来有了继母,他的体
最近,国家税务总局发布了《委托代征管理办法》,以加强税收委托代征管理,规范委托代征行为,降低征纳成本。《办法》共6章30条,全面规定了委托代征的范围和条件、委托代征协
目的构建刚地弓形虫热休克蛋白70(TgHSP70)真核表达重组质粒p3×Flag-CMW-14-TgHSP70,并在HEK 293T细胞内获得表达,为制备基因疫苗奠定基础。方法扩增TgHSP70基因,双酶切后与
原以为只有那些欧洲厂商才会对敞篷车如痴如狂到令人难以理解的地步,没想到雷克萨斯竟也“敞”出了瘾头,继SC430之后,家庭中最具运动潜质的IS系列也在2008年秋季的巴黎车展上
江淮悦悦曾在去年上海车展上首次亮相,时隔一年,经过江淮乘用车研发人员的精心雕琢,悦悦轿车更显时尚、动感,工艺也更加成熟,并预计年内上市。 JAC Yue Yue had its debut a