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目的研究神经生长因子(NGF)介导哮喘大鼠神经源性气道炎症肿瘤坏死因子α(TNF-α)mRNA的表达机制,探讨防治哮喘的新方法。方法采用放射免疫分析方法检测卵清蛋白致敏哮喘大鼠下呼吸道肺组织NGF、TNF-α的表达及抗NGF干预对其表达的影响。结果 (1)哮喘组大鼠肺组织NGF蛋白、NGF mRNA的平均灰度值分别为145±7、128±7;对照组分别为189±7、191±6;抗NGF干预组分别为156±6、139±8;3组比较差异均有统计学意义(P<0.01);(2)哮喘组大鼠TNF-α蛋白及TNF-αmRNA的灰度值分别为141±7、127±7;对照组分别为175±8、179±9;抗NGF干预组分别为164±8、155±6;3组比较差异均有统计学意义(P<0.01)。结论 NGF促进合成和释放TNF-α可能是NGF参与哮喘气道神经源性炎性反应的机制之一,抗NGF干预能够有效地将其抑制。
Objective To study the expression of tumor necrosis factor-α (TNF-α) mRNA in nerve-derived airway inflammation induced by nerve growth factor (NGF) in asthmatic rats and to explore a new method of prevention and treatment of asthma. Methods Radioimmunoassay was used to detect the expression of NGF and TNF-α in the lower respiratory tract of ovalbumin-sensitized asthmatic rats and the effect of anti-NGF treatment on its expression. Results (1) The average gray value of NGF protein and NGF mRNA in asthmatic rats were 145 ± 7 and 128 ± 7 respectively; the control group was 189 ± 7 and 191 ± 6 respectively; the anti-NGF intervention group was 156 ± 6,139 ± 8; The difference between the three groups was statistically significant (P <0.01); (2) The gray value of TNF-αmRNA and TNF-αmRNA in asthma group were 141 ± 7,127 ± 7; The control group were 175 ± 8,179 ± 9; anti-NGF intervention group were 164 ± 8,155 ± 6; 3 groups were statistically significant differences (P <0.01). Conclusion NGF can promote the synthesis and release of TNF-α, which may be one of the mechanisms of NGF involved in airway neurogenic inflammatory response in asthma. Anti-NGF intervention can effectively inhibit it.