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为明确Fas配体mRNA在正常睾丸组织及精原细胞瘤中的表达情况 ,采用原位杂交、反转录PCR(RT PCR)法检测正常睾丸组织 8例Fas配体mRNA的表达 ;采用原位杂交法检测 33例精原细胞瘤组织中的Fas配体mRNA的表达。结果RT PCR方法证明正常人睾丸组织中有Fas配体mRNA的表达 ;原位杂交法证明 8例正常睾丸组织中均有Fas配体mRNA的表达 ,且表达局限于睾丸Sertoli细胞 ,正常生殖细胞中无Fas配体mRNA的表达 ;原位杂交方法在 33例精原细胞瘤组织中检测到Fas配体mRNA表达阳性 11例 ,阳性率为 33.33% ,多呈现小片状或点状表达。提示Fas配体mRNA仅表达于人类睾丸的Sertoli细胞中 ,Fas配体mRNA在精原细胞瘤组织中的出现可能是精原细胞瘤形成及进展的原因。
In order to confirm the expression of Fas ligand mRNA in normal testis and seminoma, the expression of Fas ligand mRNA in 8 cases of normal testis tissue was detected by in situ hybridization and reverse transcription PCR (RT PCR); The expression of Fas ligand mRNA in 33 cases of seminoma was detected by hybridization. Results The RT-PCR method demonstrated the expression of Fas ligand mRNA in the testis of normal humans. In situ hybridization demonstrated the expression of Fas ligand mRNA in normal testis and the expression was limited to the Sertoli cells of the testis, normal germ cells. There was no expression of Fas ligand mRNA. In situ hybridization assay showed that 11 cases of positive Fas ligand mRNA were detected in 33 cases of seminoma, and the positive rate was 33.33%. It is suggested that Fas ligand mRNA is only expressed in Sertoli cells of human testis, and the presence of Fas ligand mRNA in seminomas may be the cause of the formation and progression of seminoma.