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为了解我国棉花耐盐相关种质资源的遗传变异,利用88对SSR引物对23份棉花耐盐材料和24份盐敏感材料进行遗传多样性分析。88个SSR位点在47份材料中共检测出338个等位基因变异,平均每个位点有3.841个;其中耐盐材料中检测出333个,盐敏感材料中检测出312个。耐盐材料的位点多态信息含量(PIC)、每个位点的有效等位基因数(Ne)、基因型多样性(H′)分别为0.613、2.929和1.083,盐敏感材料的PIC、Ne、H′分别为0.605、2.883和1.071。耐盐材料和盐敏感材料的Jaccard相似性系数分别在0.530–0.979和0.525–0.878之间,遗传相似性系数总体平均值接近,但耐盐材料的变化幅度更大。用类平均法(UPGMA)聚类将47份材料分成3个类群。总体而言,大多数材料之间的遗传相似性系数较高,表明我国陆地棉耐盐相关种质资源遗传基础狭窄。本结果为棉花耐盐育种中亲本的选配和优势组合的预测以及耐盐资源的合理利用等提供了基础资料。
In order to understand the genetic variation of salt tolerance related germplasm resources in China, 88 SSR primers were used to analyze the genetic diversity of 23 cotton salt tolerant materials and 24 salt sensitive materials. Of the 88 SSR loci, 338 alleles were detected in 47 accessions, with an average of 3.841 loci per locus. Among them, 333 were detected in salt-tolerant materials and 312 were detected in salt-sensitive materials. The polymorphic information content (PIC), the number of alleles per locus (Ne), genotype diversity (H ’) of salt tolerant materials were 0.613, 2.929 and 1.083, PIC of salt sensitive materials, Ne, H ’were 0.605, 2.83 and 1.071, respectively. The Jaccard similarity coefficients of salt-tolerant materials and salt-sensitive materials were between 0.530-0.979 and 0.525-0.878, respectively. The average of genetic similarity coefficient was close to the average, but the salt-tolerant materials changed more widely. Using UPGMA clustering, 47 materials were divided into three groups. In general, the genetic similarity coefficient between most of the materials is high, indicating that the genetic basis of salt tolerance related germplasm resources in China is narrow. The results provided the basic information for the selection of parents and the combination of superiority in cotton salt-tolerant breeding and the rational utilization of salt-tolerant resources.