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目的研究免疫性不育雄性小鼠的睾丸超微结构的特点,探讨抗精子抗体(AsAb)对睾丸生精微环境的影响。方法用同种精子及福氏佐剂免疫昆明种雄性小鼠,ELISA法检测AsAb,以此验证获得免疫性不育动物模型,同时设立对照组;解剖小鼠取其精子观察精子质量差别,透射电镜观察各组睾丸的超微结构。结果人工免疫后的雄鼠血清AsAb均为阳性,正常对照组均为阴性;模型组小鼠精子质量与对照组比较显著下降,生精上皮超微结构受损严重。结论用同种精子及福氏佐剂免疫动物,可成功制作抗精子抗体介导的免疫性不育动物模型;AsAb可通过攻击睾丸生精微环境影响雄性小鼠的生育力。
Objective To study the characteristics of testicular ultrastructure in male sterile mice and explore the effect of anti-sperm antibody (AsAb) on testicular spermatozoa microenvironment. Methods Kunming male mice were immunized with the same kind of sperm and Freund’s adjuvant, and AsAb was detected by ELISA. The immune-infertile animal model was obtained and the control group was set up. The sperm of the mice were dissected to observe the differences of sperm quality and transmission The ultrastructure of testis in each group was observed by electron microscope. Results Serum AsAb was positive in the male mice after immunization, but negative in the normal control group. The sperm quality of model mice decreased significantly compared with the control group, and the ultrastructure of the seminiferous epithelium was severely damaged. CONCLUSIONS: Immunization of animals with the same sperm and Freund’s adjuvant can successfully produce an antisperm antibody-mediated immune-infertility animal model. AsAb can affect the fertility of male mice by attacking the testicular spermatozoa.