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1998年,Yuan JL等报道了一种新TRFIA β-二酮类标记物配基——BHHCT（4,4’-双（1”,1”,1”,2”,2”,3”,3”-七氟-4”,6”-己二酮基）氯磺基-邻-三联苯）。以五氟乙基和三氟甲基替代BHHCT前体BHHT（4,4’-双（1”,1”,1”,2”,2”,3”,3”-七氟-4”,6”-己二酮基）-邻-三联苯）的七氟丙基,分别合成了BHPT（4,4’-双（1”,1”,1”,2”,2”-全氟-3”,5”-戊二酮基）邻三联苯）和BHTT（4,4’-双（1”,1”,1”-三氟-2”,4”-丁二酮基）邻三联苯）。BHTT、BHPT和BHHT的荧光寿命分别为 437、398和 341μs。在Tris-HCl缓冲液（0.1mol/L,pH=8.0）和10-6mol/LEu3+存在下,BHTT-Eu3+的荧光强度分别是BHPT-Eu3+和BHHT-Eu3+的4.3倍和11倍。滴定实
In 1998, Yuan JL et al. Reported a novel TRFIA beta-diketonate marker ligand, BHHCT (4,4’-bis (1 “, 1”, 1 “, 2”, 2 “, 3” 3 “-heptafluoro-4”, 6 “-hexadiketyl) chlorosulfo-o-terphenyl) was used instead of the BHHT precursor BHHT (4,4’-bis Heptafluoropropyl groups of 1 ”, 1“, 1 ”, 2“, 2 ”, 3“, 3 ”-heptafluoro-4“, 6 ”-hexadione) BHPT (4,4’-bis (1 “, 1”, 1 “, 2”, 2 “-perfluoro-3”, 5 “-pentadiketo) o-terphenyl) and BHTT Bis (1 ”, 1“, 1 ”-trifluoro-2“, 4 ”-diacetyl) o-terphenyl). The fluorescence lifetime of BHTT, BHPT and BHHT were 437, 398 and 341 μs, respectively. The fluorescence intensities of BHTT-Eu3 + in the presence of Tris-HCl buffer (0.1 mol / L, pH = 8.0) and 10-6 mol / LEu3 + were 4.3 and 11 times that of BHPT-Eu3 + and BHHT-Eu3 +, respectively. Titration real