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目的:研究蛋白酶活化受体4(PAR4)在小鼠背根神经节(DRG)感觉神经元的表达,及与瞬时受体电位香草酸亚型1(TRPV1)和降钙素基因相关肽(CGRP)的共存,为探讨PAR4在感觉神经伤害性刺激信号传导中的作用提供形态学依据。方法:免疫荧光组织化学双标方法结合激光共聚焦显微镜技术。结果:DRG内大量的感觉神经元表达PAR4。PAR4阳性胞体多为中、小型神经元,并可见部分阳性神经纤维。免疫荧光双标显示许多PAR4阳性神经元表达TRPV1或与CGRP共存。几乎所有的TRPV1阳性神经元均表达PAR4,大部分CGRP标记神经元呈PAR4阳性,另外还可见到许多CGRP/TRPV1双标神经元。结论:小鼠DRG初级感觉神经元广泛的表达PAR4,该受体可能通过影响TRPV1和CGRP参与伤害性刺激的调节。
AIM: To investigate the expression of PAR4 in the sensory neurons of dorsal root ganglion (DRG) of mice and its correlation with the transient receptor potential of vanilloid acid subtype 1 (TRPV1) and calcitonin gene related peptide (CGRP) ), To provide a morphological basis for exploring the role of PAR4 in the sensory nerve noxious stimulation signal transduction. Methods: Immunofluorescence histochemistry double labeling method combined with confocal laser scanning microscopy. Results: A large number of sensory neurons in the DRG expressed PAR4. PAR4 positive cell body is mostly medium and small neurons, and some positive nerve fibers can be seen. Double immunofluorescence staining showed that many PAR4-positive neurons express TRPV1 or coexist with CGRP. Almost all TRPV1-positive neurons expressed PAR4, most of the CGRP-labeled neurons were PAR4-positive, in addition to many CGRP / TRPV1 double-labeled neurons. CONCLUSION: The primary sensory neurons of DRG in mice express PAR4 extensively, which may be involved in the regulation of noxious stimulation by affecting TRPV1 and CGRP.