目的和方法：将可产生含有ＨＳＶＴＫ基因逆转录病毒的包装细胞与ＢＥＬ７４０２肝癌细胞混合后，接种于裸鼠皮下，复制肝癌模型。确定ＨＳＶＴＫ基因转移后，给予动物注射ＧＣＶ，观察ＨＳＶＴＫ／ＧＣＶ系统基因治疗实验性肝癌的在体疗效。结果：在体条件下，逆转录病毒可将ＨＳＶＴＫ基因部分转导至肝癌细胞；ＧＣＶ作用后，ＴＫ＋中瘤细胞的生长受到明显抑制；电镜观察发现，ＧＣＶ杀伤ＴＫ＋肿瘤细胞的作用主要体现在细胞核；虽然组化染色分析表明ＴＫ基因转移效率只有１０％～３０％，但测量肿瘤体积显示ＨＳＶＴＫ／ＧＣＶ系统杀伤肝癌细胞的效果依然明显，故其作用机制可与“旁观者效应”有关。结论：ＨＳＶＴＫ／ＧＣＶ“自杀”基因系统有可能成为基因治疗肝癌的有效方法 Objective and method: The packaging cells containing HSVTK gene retrovirus were mixed with BEL7402 hepatoma cells and inoculated subcutaneously in nude mice to replicate the hepatoma model. After the HSV-TK gene transfer was determined, animals were injected with GCV to observe the in vivo efficacy of HSV-TK/GCV system gene therapy for experimental liver cancer. RESULTS: Under the in vivo condition, retrovirus could partially transduce HSVTK gene into hepatoma cells; after GCV, the growth of tumor cells in TK+ cells was significantly inhibited; electron microscopy showed that the role of GCV in killing TK+ tumor cells was mainly reflected in In the nucleus; although histological staining analysis showed that the TK gene transfer efficiency was only 10% to 30%, but the measurement of tumor volume showed that the effect of HSV TK/GCV system on killing hepatoma cells was still obvious, so its mechanism of action may be related to the “bystander effect”. related. Conclusion: HSVTK/GCV suicide gene system may be an effective method for gene therapy of liver cancer