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为了解嗜水气单胞菌全基因组信息的结构和功能,对嗜水气单胞菌(Aeromonas hydrophila)AH10(CCTCC保藏号:AB2014155)进行了全基因组测序。将提取的AH10全基因组DNA进行片段化、构建文库后上机测序;将基因组组装后进行注释和比较分析。结果显示:AH10全基因组序列总长度为4.91 Mb,GC含量为61.1%,共预测到4570个基因,3351个基因具有明确的生物学功能。共找到2592个基因具有直系同源族(cluster of orthologous groups,COG)分类,1281个与代谢通路有关的基因,基因组岛31个。比较基因组学研究发现:AH10比对到SNP位点102481个,与Aeromonas hydrophila YL17基因结构共线性最好,与美国来源的嗜水气单胞菌标准菌株Aeromonas hydrophila ATCC 7966具有较近的亲缘关系,AH10的致病性与溶血素基因hly(hemolysin gene)、气溶素基因aer(aerolysin gene)、黏附素基因ahal(major adhesion gene)、丝氨酸蛋白酶基因ahp(serine protease gene)、细胞兴奋性肠毒素基因alt(cytotonic enterotoxin gene)密切相关。这些基因在嗜水气单胞菌中同源性较高。存在自体诱导物信号分子-2(autoinducer-2)与合成信号分子酶基因(signal molecule synthetase gene,ahy Ⅰ)和转录调节蛋白基因(transcriptional regular gene,Ahy R)群体感应(quorum sensing,QS)系统,不存在Ⅲ型分泌系统(type Ⅲ secretion system,TTSS)。通过对AH10全基因组序列进行较为全面的注释和比较统计分析,为嗜水气单胞菌的分子生物学研究提供理论支持。
To understand the structure and function of Aeromonas hydrophila genome-wide information, genome-wide sequencing of Aeromonas hydrophila AH10 (CCTCC accession number: AB2014155) was performed. The extracted whole genome of AH10 was fragmented, the library was constructed and sequenced. The genomes were assembled and annotated and compared. The results showed that the total length of AH10 genome was 4.91 Mb and the content of GC was 61.1%. A total of 4570 genes were predicted and 3351 genes had definite biological functions. A total of 2592 genes were found with cluster of orthologous groups (COG) classification, 1281 genes related to the metabolic pathway, and 31 genomic islands. Comparative genomics study found that: AH10 aligned to the SNP site 102481, and the Aeromonas hydrophila YL17 gene structure best linearity, and the United States of America Aeromonas hydrophila ATCC 7966 standard strain has a closer genetic relationship, The pathogenicity of AH10 is related to the hemolysin gene, the aerolysin gene, the ahal (major adhesion gene), the serine protease gene (AHP), the excitatory enterotoxin The gene alt (cytotonic enterotoxin gene) is closely related. These genes have high homology in Aeromonas hydrophila. There are autoinducer-2 and signal molecule synthetase gene (ahy Ⅰ) and transcriptional regular gene (Ahy R) quorum sensing (QS) system , There is no type III secretion system (TTSS). By comprehensively annotating and comparatively analyzing the complete genome sequence of AH10, it provides theoretical support for the molecular biology research of Aeromonas hydrophila.