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目的:制备卡铂碳包铁纳米壳聚糖微球,摸索最佳制备方案,检测该微球的各项性状,并与卡铂纯铁纳米壳聚糖微球进行比较。方法:以吸附药物的碳包铁纳米磁粉为磁性内核,壳聚糖为基质,卡铂为负载药物,采用反相微乳法制备卡铂碳包铁纳米壳聚糖微球。卡铂纯铁纳米壳聚糖微球的制备方法相似,不同的是以无吸附药物能力的纯铁纳米磁粉为磁性内核。检测和比较两种纳米药物微球的形态、粒径、磁响应性、载药量、包封率和体外释药。结果:两种药物微球的球形圆整,平均粒径210nm±26nm,粒径分布150nm-300nm,磁响应性强。碳包铁纳米微球的载药量(11.15±1.03)%,纯铁纳米微球载药量(9.21±1.10)%。碳包铁纳米微球1d、2d、3d、4d 的体外释药量分别为60%、74%、84%、92%;纯铁纳米微球1d、2d 的释药量分别为81%、91%。结论:通过活性碳吸附和物理包裹双重机制载药的卡铂碳包铁纳米壳聚糖微球不但载药量高,而且释药速度平稳。多重机制的有机结合是优化纳米微球性能的有效方法。
OBJECTIVE: To prepare carboplatin-loaded nano-chitosan microspheres and to explore the best preparation method. The properties of the microspheres were tested and compared with carboplatin-ferro-chitosan microspheres. Methods: The drug-loaded carbon-coated iron nano-magnetic powder as magnetic core, chitosan as matrix, carboplatin as loaded drug, using reverse microemulsion prepared carboplatin carbon nano-chitosan microspheres. The preparation method of carboplatin pure iron nano-chitosan microspheres is similar, except that the pure iron nano-magnetic powder with no adsorptive drug ability is a magnetic core. The morphology, particle size, magnetic responsiveness, drug loading, encapsulation efficiency and in vitro drug release of the two nanometer drug microspheres were tested and compared. Results: The two drug microspheres spherical round, the average particle size of 210nm ± 26nm, particle size distribution of 150nm-300nm, magnetic response strong. The drug-loading amount of carbon-coated iron nanospheres was (11.15 ± 1.03)%, and that of pure iron nanospheres was (9.21 ± 1.10)%. The drug release in vitro was 60%, 74%, 84% and 92% respectively in 1d, 2d, 3d and 4d of carbon-coated nano-microspheres. The release rates of pure iron nanospheres were 81% and 91% %. CONCLUSION: Carboplatin-loaded nano-chitosan microspheres loaded with dual mechanism of active carbon adsorption and physical coating not only have high loading capacity but also release rate smoothly. The organic combination of multiple mechanisms is an effective way to optimize the properties of nanospheres.