Objective: To study the effects of placenta polypeptide injection on the hemodynamics and bone metabolism after tibial fracture surgery. Methods: A prospective study was designed, and the patients with tibial fractures who received surgical treatment in our hospital between July 2015 and September 2017 were selected and randomly divided into the observation group receiving placenta polypeptide injection and the control group receiving conventional therapy. The blood viscosity as well as serum contents of thromboxane B2 (TXB2), 6-keto-prostaglandin-F1α (6-k-PGF1α) and bone metabolism markers were determined on the day and 14 days after surgery. Results: 14 days after treatment, the whole blood high shear viscosity, whole blood low shear viscosity and whole blood middle shear viscosity as well as serum TXB2, C-terminal propeptide of procollagen type I (PICP), N-terminal propeptide of procollagen type I (PINP), osteoprotegerin (OPG), osteocalcin (OC) and alkaline phosphatase (ALP) contents of both groups increased, while serum 6-k-PGF1α, β isomer of C-terminal telopeptide of type I collagen ( β-CTX), receptor activator of nuclear factor kB ligand (RANKL) and tartrate-resistant acid phosphatase 5b (TRACP5b) contents decreased, and the whole blood high shear viscosity, whole blood low shear viscosity and whole blood middle shear viscosity as well as serum TXB2, β-CTX, RANKL and TRACP5b contents of the observation group were lower than those of the control group, while serum 6-k-PGF1α, PICP, PINP, OPG, OC and ALP contents were higher than those of the control group. Conclusion: Placenta polypeptide injection therapy after tibial fracture surgery can improve hemodynamics and bone metabolism, which is beneficial to fracture healing.