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采用分离细胞培养的新生SD大鼠小脑皮质神经元，用Ara－c抑制非神经元细胞生长，研究了过氧化氢能否诱导种经元凋亡．用荧光染料Hoechst33258染色鉴别凋亡神经元，可见凋亡神经元的细胞核体积缩小至正常神经元的40％～50％，核形不规则，核内染色质块状深染．少量神经元可见到不规则的核碎片及凋亡小体。结果表明：（1）小剂量过氧化氢（50μmol／L）作用12h，可见1％～3％神经元凋亡，与对照组比较，无明显差异；24、36h凋亡神经元比对照组显著增多；48h凋亡神经元数量达高峰。（2）大剂量过氧化氢（70μmol/L）作用12h，凋亡神经元的出现明显增多，作用24、36h凋亡神经元增加均比小剂量组更显著，出现高峰更早、更高．（3）对照组随培养时间延长凋亡细胞的数量增长速度比较缓慢．以上结果提示过氧化氢能诱发小脑皮质神经元凋亡，尤以大剂量过氧化氢的诱发作用为更强．
Cultured neonatal SD rat cortical neurons with Ara-c were used to inhibit the growth of non-neuronal cells to study whether hydrogen peroxide could induce the apoptosis of neurons. Apoptotic neurons were identified by fluorescent dye Hoechst33258 staining, showing that the apoptotic neurons decreased in size from 40% to 50% of the normal neurons, with irregular nuclei and deeply stained chromatin in the nucleus. A small number of neurons can see irregular nuclear debris and apoptotic bodies. The results showed that: (1) Compared with the control group, there was no significant difference in apoptosis of neurons at 1% ~ 3% after treated with low dose hydrogen peroxide (50μmol / L) for 12h; Increased; 48h apoptotic neurons reached the peak. (2) Apoptotic neurons increased significantly at high dose of hydrogen peroxide (70μmol / L) for 12h, and the apoptotic neurons increased more significantly at 24h and 36h than those in the low dose group. The peak appeared earlier and higher. (3) The number of apoptotic cells in the control group grew more slowly with the incubation time. The above results suggest that hydrogen peroxide can induce apoptosis of cerebellar cortical neurons, especially the induction of high-dose hydrogen peroxide is stronger.