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目的建立小鼠在体肿瘤多药耐药模型。方法模拟临床以亚于治疗剂量的联合化疗PFC 方案,诱导S180腹水型小鼠4周,流式细胞仪荧光检测P170、LRP、TOPOII,建立能客观反应临床肿瘤多药耐药产生机制并适合中药干预肿瘤多药耐药研究的在体细胞模型。结果化疗诱导后昆明小鼠S180腹水肿瘤细胞P170、LRP的表达率和ROPOII活性明显提高,经传代后其表达稳定。结论联合化疗诱导建立小鼠在体肿瘤多药耐药模型是可行的。
Objective To establish a multidrug resistance model of mice in vivo. Methods To simulate the treatment of PFC with subtherapeutic doses in combination with chemotherapy, S180 ascites mice were induced for 4 weeks. Fluorescence detection of P170, LRP and TOPOII by flow cytometry was used to establish the objective mechanism of multidrug resistance in clinical tumors and to be suitable for traditional Chinese medicine Interference of tumor multidrug resistance in somatic models. Results After chemotherapy, the expression of P170, LRP and the activity of ROPOII in Kunming mice S180 ascites tumor cells were significantly increased, and the expression was stable after passage. Conclusion It is feasible to establish a multidrug resistance model of mice tumor in combination with chemotherapy.