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利用从小麦根系中分离筛选得到的对辣椒疫病有较高拮抗活性的生防细菌HG28-5菌株,通过平板对峙培养、产孢子囊和孢子萌发试验,检测其对辣椒疫霉菌的抑制作用;通过温室盆栽和小区试验,确认了对辣椒疫病的防治效果;以平板稀释培养法测定了根际定殖能力。结果表明:HG28-5显著抑制辣椒疫霉菌菌丝生长、游动孢子囊形成、游动孢子释放和休止孢子萌发;HG28-5对辣椒疫病具有明显的防治效果,在盆栽试验和小区试验中防治效果分别达到92.57%和79.96%;HG28-5在辣椒根际的定殖能力很强,用HG28-5处理辣椒种子,发芽胚根中其定殖密度为4.45×105cfu·cm-1,HG28-5处理盆栽辣椒根系,其定殖密度稳定维持在105cfu·g-1以上。利用形态特征、生理生化特性和16SrDNA序列分析,鉴定HG28-5为绿针假单胞菌。
The biocontrol bacterium HG28-5 strain isolated and screened from the root system of wheat was used to test its inhibitory effect on Phytophthora capsici by confrontation culture, sporulation and spore germination test. Pot experiments in greenhouse and plots were conducted to confirm the control effect on pepper blight. The rhizosphere colonization ability was determined by plate dilution culture method. The results showed that HG28-5 significantly inhibited the mycelial growth, zoospore formation, zoospore release and resting spore germination. Phytophthora capsici HG28-5 had significant control effects on pepper blight and its control in pot experiment and plot test The results showed that HG28-5 had strong colonization ability in pepper rhizosphere. HG28-5 was used to treat pepper seeds with germination density of 4.45 × 105 cfu · cm-1 in germinating radicle, HG28- 5 treatment of potted pepper roots, its colonization density remained stable above 105cfu · g-1. Using morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis, HG28-5 was identified as Pseudomonas aeruginosa.