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目的:比较在南京引种栽培后承德、成都、太原、桐城四个不同居群薏苡种仁脂肪酸成分的差异。方法:薏苡仁分别用乙醚超声提取,提取物甲酯化后进行气相色谱-质谱联用(GC-MS)分析,并利用NIST谱库检索鉴定化合物,用归一化法计算各化合物的相对百分含量。结果:从不同居群薏苡种仁的乙醚提取物中鉴定了14种成分,其中有6种成分为4个居群所共有,分别是壬二酸、棕榈酸、亚油酸、油酸、11-十八碳烯酸及硬酯酸。结论:不同居群薏苡仁脂肪酸成分种类和含量都有一定程度的变化。承德居群脂肪酸成分的种类要多于其他居群,主要成分的含量也略高,可作为优质种源加以进一步选育研究。
OBJECTIVE: To compare the differences in fatty acid composition in four different populations of Chengde, Chengdu, Taiyuan and Tongcheng after introduction and cultivation in Nanjing. Methods: The barley kernels were extracted with ethyl ether ultrasonically, and the extracts were methylated and analyzed by gas chromatography-mass spectrometry (GC-MS). The compounds were identified by NIST library search, and the relative hundred of each compound were calculated by the normalized method. Content. Results: 14 components were identified from the ether extracts of different populations of seed kernels, of which 6 components were shared by 4 populations, which were sebacic acid, palmitic acid, linoleic acid, oleic acid, 11 - octadecenoic acid and stearic acid. Conclusion: There are some changes in the types and contents of fatty acids in different populations. Chengde population has more species of fatty acids than other populations, and the content of main components is slightly higher. It can be used as a high-quality seed source for further breeding research.