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目的：了解ＩＦＮγ对持续肝外性淤胆所致肝纤维化的作用。方法：制作大鼠持续肝外性淤胆模型，用药组于胆管结扎同时每日肌注ＩＦＮγ５×１０４Ｕ，用药２周；对照组则仅行胆管结扎而不用药。２周后分别测定血清生化指标、肝纤维化指标及肝能量代谢有关指标。结果：ＩＦＮγ可改善血清生化指标（Ｐ＜０．０１或Ｐ＜０．０５）。在组织学表现上，用药组肝纤维化程度显著低于单纯结扎组（Ｐ＜０．０１）。用药组大鼠淤胆后肝羟脯氨酸及血清透明质酸水平升高明显受抑（Ｐ＜０．０１）。肝ＡＴＰ及ＣＰ测定显示淤胆合并用药时两者均保持于较高水平。结论：ＩＦＮγ可有效抑制大鼠持续肝外性淤胆所致肝纤维化
Objective: To understand the effect of IFNγ on liver fibrosis induced by persistent extrahepatic cholestasis. Methods: The model of persistent liver cholestasis in rats was made. The rats in the medication group were intramuscularly injected with IFNγ5 × 104U daily for 2 weeks while those in the control group were given only biliary ligation without medication. Serum biochemical indexes, liver fibrosis indexes and liver energy metabolism indexes were measured after 2 weeks. Results: IFNγ could improve serum biochemical parameters (P <0.01 or P <0.05). In histological manifestations, the degree of hepatic fibrosis in the treatment group was significantly lower than that in the simple ligation group (P <0.01). The levels of hydroxyproline and serum hyaluronic acid were significantly inhibited in the rats treated with cholesty (P <0.01). Liver ATP and CP measurements showed that both cholestasis combined with medication were maintained at a high level. Conclusion: IFNγ can effectively prevent liver fibrosis caused by persistent extrahepatic cholestasis in rats