论文部分内容阅读
目的：观察氨茶碱和地塞米松对小鼠脾淋巴细胞凋亡的调节作用。方法：采用流式细胞技术对比了它们对正常鼠及卵蛋白致敏小鼠脾淋巴细胞凋亡的作用。结果：氨茶碱及地塞米松可诱导体外培养的正常小鼠淋巴细胞凋亡，明显抑制体外培养的致敏鼠淋巴细胞的凋亡。致敏鼠淋巴细胞的凋亡明显多于对照鼠，在体应用氨茶碱（１００ｍｇ／ｋｇ）使致敏淋巴细胞凋亡进一步增加，而在体应用地塞米松（４０ｍｇ／ｋｇ）明显抑制致敏鼠淋巴细胞的凋亡。氨茶碱体外对在体应用氨茶碱处理的鼠淋巴细胞的凋亡有抑制作用，地塞米松体外对在体应用地塞米松处理的鼠淋巴细胞的凋亡无明显影响。结论：氨茶碱和地塞米松对淋巴细胞凋亡具有重要的调节作用，两者的作用机制可能有别。
Objective: To observe the effect of aminophylline and dexamethasone on the apoptosis of mouse splenic lymphocytes. Methods: Flow cytometry was used to compare their effects on the apoptosis of splenic lymphocytes in normal and ovalbumin-sensitized mice. Results: Aminophylline and dexamethasone could induce lymphocyte apoptosis of normal mice in vitro and significantly inhibit the apoptosis of sensitized murine lymphocytes cultured in vitro. Apoptosis of sensitized murine lymphocytes was significantly more than that of control mice. Aminophylline (100 mg / kg) in vivo sensitized lymphocyte apoptosis to further increase, while in vivo dexamethasone (40 mg / kg) significantly inhibited Apoptosis of Sensitive Rat Lymphocytes. Aminophylline inhibited the apoptosis of lymphocytes in vivo treated with aminophylline in vitro. Dexamethasone had no significant effect on the apoptosis of lymphocytes in vivo treated with dexamethasone in vivo. Conclusion: Aminophylline and dexamethasone have an important regulatory effect on lymphocyte apoptosis, and their mechanism of action may be different.