目的建立降糖类中药制剂中同时检测盐酸二甲双胍等6种化学药品的HPLC。方法采用Hypersil BDS C18色谱柱(200 mm×4.6 mm,5μm),以离子对试液(取庚烷磺酸钠0.5 g,加三乙胺1.4 mL,加水至1 000 mL,用磷酸调节pH值至3.5±0.05)-甲醇为流动相,梯度洗脱;流速为1.0 mL.m in-1,检测波长为233 nm。结果6种化学药品均能得到较好的分离,且在一定的进样量范围内呈现良好的线性。结论本法快速、简便,能同时对6种化学药品进行定性、定量分析,可用于鉴定降糖中药制剂中是否添加化学药品。 Objective To establish a HPLC method for simultaneous determination of six chemicals, including metformin hydrochloride in hypoglycemic traditional Chinese medicine preparations. Methods Hypersil BDS C18 column (200 mm × 4.6 mm, 5 μm) was used. The ion pair solution (0.5 g heptane sulfonate, 1.4 mL triethylamine and water to 1000 mL) was adjusted with phosphoric acid To 3.5 ± 0.05) -Methanol as the mobile phase and gradient elution. The flow rate was 1.0 mL.m in-1 and the detection wavelength was 233 nm. Results All six chemicals were well separated and showed a good linearity within a certain range of injection volume. Conclusion The method is rapid and simple, and can be used for the qualitative and quantitative analysis of six kinds of chemicals at the same time. It can be used to identify whether to add chemicals in the preparation of hypoglycemic Chinese medicine.