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目的:探讨应用外源性bFGF对实验断层皮片组织变化的影响。方法:建立豚鼠断层皮片自体移植模型。A组为对照组,B、C、D组为实验组,分别给予bFGF300Au/ml、600Au/ml、900Au/ml,术后第1、2、4周切取皮片,行常规组织学、电镜观察并计数真皮毛细血管数量与成纤维细胞密度值。结果:术后第1周,除A组外,各组均见毛细血管、成纤维细胞及胶原纤维增生,D组更显。术后第2周,B、C、D组毛细血管、成纤维细胞及胶原纤维增生。仍以D组更显,术后第4周,B、C、D组轻度毛细血管、成纤维细胞及胶原纤维增生,各组差别不明显。结论:外源性bFGF具有促进实验断层皮片毛细血管、成纤维细胞及胶原纤维增生的作用,并呈剂量依赖性。bFGF有利于皮片的念合及创面修复。
Objective: To investigate the effect of exogenous bFGF on the changes of experimental skin tissue. Methods: A guinea pig autotransplantation model was established. Group A was the control group, Group B, C and D were experimental group, bFGF300Au / ml, 600Au / ml and 900Au / ml respectively. The number of dermal capillaries and fibroblast density were counted. Results: In the first week after operation, except group A, the proliferation of capillary vessels, fibroblasts and collagen fibers were observed in all groups, and the group D was more obvious. At the second week after operation, the capillaries, fibroblasts and collagen fibers in groups B, C and D proliferated. Still more in group D, 4 weeks after operation, B, C, D group mild capillaries, fibroblasts and collagen fibers proliferation, the difference was not obvious in each group. Conclusion: Exogenous bFGF can promote the proliferation of capillaries, fibroblasts and collagen fibers in experimental dermal grafts in a dose-dependent manner. bFGF is conducive to the concept of skin flap and wound repair.