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目的:研究益血口服液对成人细胞免疫功能及对小鼠骨髓造血细胞有丝分裂的影响。方法:将不同剂量益血宁口服液作用于12名健康男性受试者的外周血淋巴细胞,以氢化可的松为阳性对照,经淋巴细胞转化技术(LTT)处理,以形态学方法考察淋巴细胞转化百分率(LTP);以48只昆明种小鼠分为3组,以丝裂霉素C(MMC)为阳性对照,给药10d后取双侧股骨骨髓细胞,镜检计算其中的有丝分裂指数;同时考察了益血口服液中Fe2+,Zn2+,Cu2+含量。结果:益血口服液对提高细胞免疫功能在此范围有量效关系,对正常状态的动物有促进骨髓造血细胞有丝分裂的作用。结论:本品具有提高细胞免疫功能及促进骨髓造血细胞有丝分裂的作用,Fe2+,Zn2+离子含量较高,可能是其对营养性缺铁性贫血具有治疗效果的药理学基础。
Objective: To study the effect of Yixue Oral Liquid on cellular immune function and mitosis in mouse bone marrow hematopoietic cells. Methods: Peripheral blood lymphocytes from 12 healthy male subjects were treated with different doses of YKLK. Hydrocortisone was used as a positive control and treated by lymphocyte transformation (LTT) 48 Kunming mice were divided into 3 groups. Mitomycin C (MMC) was used as a positive control. Bilateral femur bone marrow cells were harvested 10 days after administration. The mitotic index At the same time, the contents of Fe2 +, Zn2 + and Cu2 + in Yixue oral solution were also investigated. Results: Yixue oral liquid has the dose-effect relationship in improving cellular immune function in this range, and can promote the mitosis of bone marrow hematopoietic cells in normal animals. Conclusion: The product has the function of increasing cellular immunity and promoting the mitosis of bone marrow hematopoietic cells. The high content of Fe2 + and Zn2 + ions may be the pharmacological basis for its therapeutic effect on nutritional iron deficiency anemia.