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健康中国人外周血白细胞,经新城鸡瘟病毒诱导表达人α2b-干扰素 mRNA,用总RNA 提取试剂盒提取总 RNA,利用逆转录-多聚酶链反应技术扩增编码人α2b-干扰素的 cDNA序列,并将其克隆人 PUC18载体。本文 DNA 序列分析结果证实所克隆的 cDNA 是人α2b-干扰素基因。
Human peripheral blood leukocytes from healthy Chinese were induced to express human α2b-interferon mRNA by the Newcastle disease virus. The total RNA was extracted by total RNA extraction kit and the cDNA sequence encoding human α2b-interferon was amplified by reverse transcription-polymerase chain reaction (PCR) And cloned into human PUC18 vector. The results of DNA sequence analysis in this paper confirm that the cloned cDNA is a human α2b-interferon gene.