用胶原酶孵育后机械分离15只小鸡的听毛细胞，平均每侧可分离428个。对其中5只鸡的215个活性良好的单离毛细胞进行了形态学测量，其中高毛细胞164个，长20．22±3．32μm，宽9．93±2．01μm，矮毛细胞30个，长10．97±2．29μm，宽14．8±2．29μm，中间型毛细胞21个，长宽相等，均为11.93±1.74μm。用双醋酸荧光素-碘化丙啶对另外5只鸡单离毛细胞进行染色，在荧光显微镜下观察细胞的变性过程。鸡断头3h内，活性良好的细胞着非常明亮的绿色荧光，到5h，活性细胞呈现不清晰的绿影。实验结果表明，判断单离毛细胞的活性，应将形态学观察标准与细胞染色结合起来。 After incubation with collagenase, 15 chickens were mechanically isolated from the hair cells, with an average of 428 isolated on each side. 215 of 5 chickens were morphologically measured with well-isolated, free-standing hair cells, including 164 high hair cells, 20.22 ± 3.32 μm in length, 9.93 ± 2.01 μm in width, 30 A length of 10.97 ± 2.29μm, a width of 14.8 ± 2.29μm, 21 intermediate hair cells, the same length and width, were 11.93 ± 1.74μm. The other 5 chickens were stained with fluorescein-propidium iodide, and the degeneration of cells was observed under a fluorescence microscope. Within 3h, the well-behaved cells had a very bright green fluorescence. By 5h, the active cells showed an unclear green shadow. The experimental results show that, judging the activity of isolated hair cells, morphological observation standards should be combined with cell staining.