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目的 建立高低不同转移特性人成骨肉瘤MG 63亚克隆细胞系 ,为研究成骨肉瘤转移机制提供较好的实验模型。方法 通过体外培养和裸鼠体内移植 ,初步分离并建立了 2个亚克隆细胞系A1和A2 ,并利用细胞电泳、细胞增殖、琼脂克隆形成、体外侵袭实验、裸鼠体内异位和原位移植对两者的生物学特性进行比较、分析和鉴定。结果 A1和A2的电泳率、琼脂克隆形成能力、体外侵袭能力、自发性肺转移率分别为 (1.0 8± 0 .12 ) μm ,(0 .64± 0 .13 ) μm ;2 1.0± 2 .3 ,9.5±2 .9;186.0± 16.7,84.0± 12 .6;10 0 .0 0 % ,6.67% ,A1均明显高于A2 ,两者的差异有统计学意义(P <0 .0 1)。结论 高低不同转移特性的人成骨肉瘤MG 63亚克隆细胞系的建立 ,能为成骨肉瘤转移机制的研究提供较理想的实验模型。
OBJECTIVE: To establish a subclone cell line of human osteosarcoma MG 63 with different metastatic potential, and to provide a better experimental model for studying the metastatic mechanism of osteosarcoma. Methods Two subclone cell lines, A1 and A2, were initially isolated and cultured in vitro and transplanted in nude mice. Cell electrophoresis, cell proliferation, agarose colony formation, in vitro invasion assay, ectopic and orthotopic transplantation The biological characteristics of the two were compared, analyzed and identified. Results The electrophoresis rates of A1 and A2, the ability of agar colony formation, the invasiveness in vitro and the rate of spontaneous pulmonary metastasis were (1.08 ± 0.12) μm, (0.64 ± 0.13) μm and 2 1.0 ± 2, respectively. 3, 9.5 ± 2 .9; 186.0 ± 16.7, 84.0 ± 12.6; 100.0%, 6.67%, A1 were significantly higher than A2, the difference between the two was statistically significant (P0.01 ). CONCLUSION: The establishment of subcellular clonal sublineage of human osteosarcoma MG 63 with different metastatic characteristics can provide an ideal experimental model for studying the metastatic mechanism of osteosarcoma.