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目的:采用高效液相色谱法测定安胎丸中黄芩苷的含量。方法:高效液相色谱法,Kromasil C18(5μm,250 mm×4.6 mm),0.1%磷酸溶液-甲醇(45∶55)为流动相,流速1 mL.min-1,检测波长280 nm,柱温25℃。结果:黄芩苷在5.096～30.576μg线性关系良好,r=0.999 9,回归方程Y=72 808.17X-41 785.27,仪器精密度的RSD(n=5)为0.03%;方法重复性的RSD(n=6)为0.5%。平均回收率(n=6)为100.23%,RSD1.7%。结论:本法快速简便,重复性好,测定准确灵敏。
Objective: To determine the baicalin content in Antaiwan by HPLC. Methods: High performance liquid chromatography, Kromasil C18 (5μm, 250 mm×4.6 mm), 0.1% phosphoric acid solution-methanol (45:55) as mobile phase, flow rate 1 mL.min-1, detection wavelength 280 nm, column temperature 25°C. Results: Baicalin has a good linear relationship between 5.096 and 30.576 μg, r=0.999 9, regression equation Y=72 808.17X-41 785.27, RSD (n=5) of instrument precision is 0.03%; method reproducibility RSD (n =6) is 0.5%. The average recovery rate (n=6) was 100.23% and RSD 1.7%. Conclusion: This method is rapid, simple, reproducible, and accurate and sensitive.