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为了探索丙型肝炎病毒(HCV)的母-婴传播的危险性,作者对116名HCV阳性母亲所生的婴儿进行了随访研究,其中22位母亲同时感染有人类免疫缺陷病毒(HIV)。婴儿出生时做血液检查和实验室及临床评估,约每3个月作一次这样的随访调查,1年以后改为约6个月一次。抗HCV抗体的检查用EIA,反应可重复的样本用RIBA法证实,病毒RNA的检测用套式逆转录PCR法,引物来源于HCV基因组5’端非编码区。在母亲分娩时进行,婴儿至少做两次。
To explore the risk of mother-to-child transmission of hepatitis C virus (HCV), the authors conducted a follow-up study of 116 infants born to HCV-positive mothers, of whom 22 were infected with human immunodeficiency virus (HIV). Blood tests and laboratory and clinical evaluations of infants at birth are made. The follow-up survey is made about every 3 months and changed to about 6 months after 1 year. Anti-HCV antibodies were detected by EIA. Reproducible samples were confirmed by RIBA. The viral RNA was detected by nested reverse transcription PCR. The primers were derived from the 5 ’non-coding region of HCV genome. When the mother is giving birth, the baby should be done at least twice.