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目的:建立愈风宁心片的特征图谱,提高其质量控制水平。方法:以葛根素为参照峰,采用CAPCELL PAKMGⅡ、AgilentZORBAX SB或Kromasil100-5 C18色谱柱,以甲醇(A)-水(B)为流动相,进行梯度洗脱(0～35 min,15%～30%A;35～60 min,30%～90%A);流速1.0 mL.min-1;检测波长:250 nm。结果:愈风宁心片特征图谱有9个共有峰,方法学考察结果良好,该方法已推广至系列品种。结论:采用HPLC特征图谱可实现全面和整体的评价,为有效提高愈风宁心片质量控制水平提供依据。
Objective: To establish the characteristic map of Yufengning Tablet and improve its quality control. Methods: With puerarin as the reference peak, gradient elution was performed with CAPCELL PAKMGⅡ, Agilent ZORBAX SB or Kromasil100-5 C18 column with methanol (A) -water (B) 35 min, 15% -30% A, 35-60 min, 30% -90% A), flow rate 1.0 mL.min-1, detection wavelength 250 nm. Results: There were 9 common peaks of Yufengning tablets, and the methodological results were good. The method has been extended to a series of varieties. Conclusion: The comprehensive and overall evaluation can be achieved by HPLC profiling, which provides the basis for effectively improving the quality control of Yufengning Tablet.