论文部分内容阅读
目的探讨褪黑素受体激动剂Neu-P11对胰岛素抵抗3T3-L1脂肪细胞IRS-1和GLUT-4表达的影响。方法用高糖高胰岛素作用3T3-L1脂肪细胞24 h,建立胰岛素抵抗细胞模型。分别采用褪黑素、Neu-P11、褪黑素+luzindole(褪黑素受体拮抗剂)和Neu-P11+luzindole处理细胞,并以未加药细胞作为对照。用葡萄糖氧化酶法检测培养液中的糖含量并计算糖消耗量,用蛋白质印迹分析检测细胞内IRS-1、GLUT-4蛋白表达变化。结果胰岛素抵抗细胞用褪黑素和Neu-P11处理后,糖消耗量较未加药对照组升高(P<0.05),GLUT-4、IRS-1的蛋白表达也增高(P<0.05);褪黑素和Neu-P11分别与luzindole联合作用于细胞后,细胞的上述蛋白表达较单用褪黑素组和单用Neu-P11组降低(P<0.05),而与未加药对照组相比差异无统计学意义。结论褪黑素受体激动剂Neu-P11可提高胰岛素抵抗脂肪细胞的胰岛素敏感性,增加糖消耗量,这种作用可能与上调IRS-1、GLUT-4蛋白的表达有关。
Objective To investigate the effect of melatonin receptor agonist Neu-P11 on IRS-1 and GLUT-4 expression in insulin resistance 3T3-L1 adipocytes. Methods 3T3-L1 adipocytes were treated with high glucose and high insulin for 24 h to establish a model of insulin resistance. The cells were treated with melatonin, Neu-P11, melatonin + luzindole (melatonin receptor antagonist) and Neu-P11 + luzindole, respectively. Glucose oxidase method was used to detect the sugar content in the culture medium and the sugar consumption was calculated. The changes of IRS-1 and GLUT-4 protein expression were detected by Western blotting. Results After treatment with melatonin and Neu-P11, the glucose consumption of insulin-resistant cells was higher than that of the untreated control group (P <0.05) and the protein expression of GLUT-4 and IRS-1 was also increased (P <0.05) Melatonin and Neu-P11 combined with luzindole, respectively, decreased the expression of these proteins (P <0.05) in the cells compared with the melatonin group and the Neu-P11 alone group, but not with the untreated control group The difference was not statistically significant. Conclusions Neu-P11, a melatonin receptor agonist, increases insulin sensitivity and increases glucose consumption in insulin-resistant adipocytes, which may be related to up-regulation of IRS-1 and GLUT-4 protein expression.