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目的:观察人参皂苷Rg1诱导人神经干细胞(neural stem cells of human,hNSCs)的功能表达。方法:采用全细胞膜片钳技术分析人参皂苷Rg1诱导hNSCs分化7 d时,神经元样细胞的膜电生理特性以及钠、钾离子通道的功能表达。结果:人参皂苷Rg1(20 mg·L-1)诱导hNSCs分化7 d时,神经元样细胞的膜静息电位为(-45.70±2.63)mV,膜电容为(26.89±1.91)pF,膜输入阻抗为(877.51±20.44)MΩ(与对照组相比均P<0.05);电压依赖性的快速激活、快速失活的内向Na+电流,检出率50%,平均峰值为(711.48±158.03)pA(与对照组相比P<0.05);外向K+电流的主要成分是快速激活快速失活的瞬时外向K+电流和延迟整流外向K+电流,其平均峰值为(1 070.42±177.18)pA(与对照组相比P<0.05)。结论:人参皂苷Rg1可以促进hNSCs功能表达和成熟。
Objective: To observe the functional expression of ginsenoside Rg1 on human neural stem cells (hNSCs). Methods: Whole-cell patch-clamp technique was used to analyze the electrophysiological characteristics of neuron-like cells and the functional expression of sodium and potassium channel in 7 h after ginsenoside Rg1 induced differentiation of hNSCs. Results: The resting membrane potential of neuron-like cells was (-45.70 ± 2.63) mV and the membrane capacitance was (26.89 ± 1.91) pF at 7 days after induction of hNSCs by ginsenoside Rg1 (20 mg · L-1) The impedance was (877.51 ± 20.44) MΩ (P <0.05 compared with the control group). The voltage-dependent rapid activation, rapid inactivation of inward Na + current, the detection rate was 50%, the average peak was (711.48 ± 158.03) pA (P <0.05 compared with the control group). The main components of extrinsic K + current were rapid outward activation K + current and delayed rectifier outward K + current. The average peak value was (1070.42 ± 177.18) pA Compared to P <0.05). Conclusion: Ginsenoside Rg1 can promote the functional expression and maturation of hNSCs.