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本实验将从日本血吸虫成虫分离纯化出的２８ｋＤａ、３１／３２ｋＤａ及９７ｋＤａ蛋白混合组成多价分子疫苗免疫小鼠，再行攻击感染，观察其保护性免疫力。结果：疫苗加佐剂组成虫减虫率、肝组织减卵率及肠壁组织减卵率分别为５０．３％、７２．０％和８１．５％，均显著高于对照组（Ｐ＜０．０１）；实验组肝虫卵肉芽肿较对照组炎症反应程度轻，虫卵肉芽肿周长及面积均低于对照组（Ｐ＜０．０１）。结果提示，由日本血吸虫成虫２８ｋＤａ、３１／３２ｋＤａ及９７ｋＤａ蛋白联合组成的多价分子疫苗，具有较高的减虫率、减卵率及抑制虫卵肉芽肿形成的作用，有一定的应用前景
In this study, we isolated and purified 28kDa, 31 / 32kDa and 97kDa proteins from adult Schistosoma japonicum to form multivalent molecular vaccine to immunize mice and then attacked the infected mice to observe their protective immunity. Results: The worm reduction rate, the rate of reduction of liver tissue and the rate of reduction of intestinal wall tissue were 50.3%, 72.0% and 81.5% respectively, which were significantly higher than those of the control group (P <0. .01). The hepatic worm egg granuloma in the experimental group was lighter than that in the control group, and the perimeter and area of the egg granuloma were both lower than those in the control group (P <0.01). The results suggest that the multivalent molecular vaccine consisting of 28kDa, 31 / 32kDa and 97kDa proteins of adult Schistosoma japonicum has a higher application rate of worm reduction, egg reduction and inhibition of egg granuloma formation