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目的:研究转化生长因子β1(TGF-β1)第一外显子区+869T/C、+915G/C基因多态性及与环境易感因素的交互作用对高血压的影响。方法:采用病例对照研究的方法,用PCR-RFLP和基因测序进行基因分型。结果:+869 C等位基因及CC基因型频率在EH组中高于对照组(61.1%vs 51.2%、41.34%vs 28.57%,P<0.05),C等位基因携带者EH患病风险是T等位基因携带者的1.497倍(P=0.00);+869CC基因型与吸烟(OR=2.17,P=0.00)、饮酒(OR=2.28,P=0.01)、超重(OR=4.57,P=0.00)、肥胖(OR=26.14,P=0.00)、蔬菜摄入不足(OR=2.09,P=0.00)有正交互作用;+915G/C等位基因G、C频率依次为99.7%、0.3%(P>0.05)。结论:+869位点CC基因与蔬菜摄入不足、吸烟、饮酒、超重、肥胖共同增加了EH患病风险。
OBJECTIVE: To study the effect of the interaction between polymorphisms of + 869T / C and + 915G / C in exon 1 of transforming growth factor β1 (TGF-β1) and environmental factors on hypertension. METHODS: Genotyping was performed using PCR-RFLP and gene sequencing using a case-control study. Results: The frequencies of +869 C allele and CC genotype in EH group were higher than those in control group (61.1% vs 51.2%, 41.34% vs 28.57%, P <0.05). The risk of EH in C allele was T (OR = 2.28, P = 0.01), overweight (OR = 4.57, P = 0.00), alcohol consumption (OR = 2.17, P = 0.00) ), Obesity (OR = 26.14, P = 0.00), and insufficient vegetable intake (OR = 2.09, P = 0.00). The frequencies of G and C alleles of 915G / C allele were 99.7% and 0.3% P> 0.05). Conclusion: Ingestion of CC gene and vegetables at +869 site is inadequate. Smoking, drinking, overweight and obesity increase the risk of EH.