论文部分内容阅读
为了研究铅和乙醇的联合作用对大鼠精子的影响,选取健康雄性Wistar大白鼠54只,体重(207±17)g,采用2×3析因设计方法随机分为9组,每组6只,A～H组分别给予醋酸铅11m g/kgBW 和醋酸铅22m g/kgBW、乙醇0.9g/kgBW、乙醇1.8g/kgBW、醋酸铅11m g/kgBW+ 乙醇0.9g/kgBW、醋酸铅11m g/kgBW+ 乙醇1.8g/kgBW、醋酸铅22m g/kgBW+ 乙醇0.9g/kgBW、醋酸铅22m g/kgBW+ 乙醇1.8g/kgBW,对照组给等量蒸馏水。每天1次,每周5次灌胃,连续4周,染毒期满断头处死,取附睾尾做精子分析。与各单独染毒组比较,联合染毒组的精子计数和精子活动率显著降低,精子畸变率、附睾/体重比和血铅水平显著增高(P< 0.01,P< 0.05),析因分析表明铅和乙醇有交互作用(P< 0.05), 即铅和乙醇联合呈现增毒作用;逐步回归分析血铅与精子畸变率呈正相关,与精子活动率呈负相关。结果提示铅和乙醇对雄性大鼠的精子质量有联合增毒作用,严重影响精液质量,损害雄性生殖功能。其损伤机制可能与联合染毒后乙醇促进了铅的吸收或释放致血铅增高,加重了生精细胞的损伤有关
In order to study the effects of lead and ethanol on spermatogenesis in rats, 54 healthy male Wistar rats weighing 207 ± 17 g were randomly divided into 9 groups with 2 × 3 factorial design, with 6 rats in each group . The lead acetate 11m g / kg BW and lead acetate 22m g / kgBW, ethanol 0.9g / kgBW, ethanol 1.8g / kgBW, lead acetate 11m g / kgBW + ethanol 0.9g / kgBW and lead acetate 11m g / kgBW + / kgBW, lead acetate 22m g / kgBW + ethanol 0.9g / kgBW, lead acetate 22m g / kgBW + ethanol 1.8g / kgBW, the control group to the same amount of distilled water. 1 day, 5 times a week gavage for 4 weeks, the decapitation after the expiration of the poisoning, take epididymal sperm analysis. The sperm count and sperm motility of the combined exposure groups were significantly lower than those of the control groups (P <0.01, P <0.05), and the sperm abnormality rate, epididymal / body weight ratio and blood lead level were significantly increased There was interaction between lead and ethanol (P <0.05), that is, the combination of lead and ethanol increased the toxic effect. The stepwise regression analysis showed that blood lead was positively correlated with sperm abnormality rate and negatively correlated with sperm activity rate. The results suggest that lead and ethanol have a joint toxic effect on the sperm quality of male rats, which will seriously affect the quality of semen and impair the male reproductive function. The mechanism of injury may be related to the combination of alcohol and ethanol to promote lead absorption or release lead to increased blood lead, aggravating the damage of spermatogenic cells