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将甲型肝炎病毒基因全部开放读码框架cDNA连接于痘苗病毒P11启动子下游,插入痘苗病毒天坛株基因组Hind Ⅲ M片段,获得重组病毒VMS11HAV25。重组病毒能表达甲肝全部结构蛋白。免疫家兔能产生甲肝中和抗体。
The full open reading frame cDNA of the Hepatitis A virus gene was ligated to the downstream of P11 promoter of vaccinia virus and the Hind Ⅲ M fragment of the vaccinia virus Tiantan strain was inserted into the recombinant virus VMS11HAV25. Recombinant virus can express all the structural proteins of hepatitis A. Immunized rabbits can produce hepatitis A neutralizing antibodies.