论文部分内容阅读
目的:观察并分析蛋白酶激活受体-1(proteinase activated receptor-1,PAR-1)基因在口腔舌鳞癌(oral tongue squamouscell carcinoma,OTSCC)中的表达情况及其临床意义,探讨PAR-1作为OTSCC潜在治疗靶点的可能性。方法:用实时定量RT-PCR检测15例OTSCC组织及癌旁舌组织中PAR-1 mRNA的表达,用免疫组化检测PAR-1蛋白在109例OTSCC组织及其癌旁舌组织中的表达情况。结果:PAR-1 mRNA及蛋白在OTSCC组织中的表达明显高于其在癌旁舌组织中的表达(P<0.001);PAR-1蛋白高表达出现于OTSCC肿瘤直径较大和TNM分期晚的患者,在统计学上有差异(P<0.05);随着OTSCC分化程度的降低、淋巴结转移,PAR-1蛋白表达也随之增高,但无明显统计学差异(P>0.05);PAR-1蛋白在OTSCC组织中的表达与患者性别、年龄无关。经Kaplan-Meier法生存曲线和COX比例风险模型分析,结果显示PAR-1高表达和TNM分期晚是OTSCC患者预后不良的独立因素。结论:PAR-1高表达与OTSCC的不良预后有关,可作为临床预后危险因素之一,也可作为靶向治疗OTSCC的潜在靶点。
OBJECTIVE: To observe and analyze the expression of PAR-1 gene in oral tongue squamous cell carcinoma (OTSCC) and its clinical significance. To investigate the role of PAR-1 in the pathogenesis of oral tongue squamous cell carcinoma (OTSCC) OTSCC potential therapeutic targets. Methods: The expression of PAR-1 mRNA in 15 OTSCC tissues and adjacent non-cancerous tongue tissues was detected by real-time quantitative RT-PCR. The expression of PAR-1 protein in 109 OTSCC tissues and adjacent non-cancerous tongue tissues was detected by immunohistochemistry . Results: The expression of PAR-1 mRNA and protein in OTSCC tissues was significantly higher than that in adjacent non-cancerous tissues (P <0.001). The high expression of PAR-1 protein was found in OTSCC patients with large tumor diameter and late TNM stage (P <0.05). With the decrease of OTSCC differentiation, lymph node metastasis, PAR-1 protein expression also increased, but no significant difference (P> 0.05); PAR-1 protein The expression in OTSCC tissues has nothing to do with patient’s sex and age. Kaplan-Meier survival curves and COX proportional hazards model analysis showed that the high expression of PAR-1 and TNM staging is an independent prognostic factor for poor prognosis in patients with OTSCC. Conclusion: The high expression of PAR-1 is associated with the poor prognosis of OTSCC, which may serve as one of the risk factors of clinical prognosis and as a potential target of targeted therapy for OTSCC.