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目的了解雷丸及吡喹酮对猬裂头蚴疗效的影响。方法 (1)从黑斑蛙体内检获裂头蚴。将112只昆明小鼠分成14组(A-I),每只小鼠经口感染裂头蚴5条,每组8只小鼠,每组共感染裂头蚴40条。(2)感染后1周,A-C组小鼠分别用吡喹酮灌胃治疗1个疗程(1次/d×3 d),吡喹酮1个疗程的总剂量分别为1 500 mg/kg、3 000mg/kg和6 000 mg/kg;D-F组分别用雷丸灌胃治疗1个疗程(1次/d×3 d),雷丸1个疗程的总剂量分别为1 200mg/kg、2 400 mg/kg和4 800 mg/kg。(3)H-J组和K-M组小鼠分用吡喹酮和雷丸治疗一个疗程后,间隔7 d,再治疗1个疗程,H-J组的总剂量同A-C组,K-M组的总剂量同D-F组;G组和N组小鼠仅灌服生理盐水,分别作为A-F组和H-M组小鼠的对照组。所有处理组及对照组在疗程结束后1周剖杀,分别计数每组小鼠的裂头蚴检获数及减虫率。结果 (1)1个疗程治疗:A组及B组小鼠与G对照组相比,裂头蚴的减虫率差异无统计学意义(P>0.05);C组小鼠裂头蚴的平均检获数为2.13条,裂头蚴的减虫率为41.32%,与G对照组相比,差异有统计学意义(P<0.05);D组、E组及F组小鼠裂头蚴的减虫率分别为48.21%、51.79%和44.90%,与G对照组相比,差异均有统计学意义(P<0.05)。(2)2个疗程的治疗:H、I和J组与N对照组相比,裂头蚴的减虫率差异无统计学意义(P>0.05);K、L和M组小鼠裂头蚴的减虫率分别为48.22%、55.62%和59.17%,与N对照组相比,差异均有统计学意义(P<0.05),雷丸各治疗组之间裂头蚴的减虫率差异无统计学意义(P>0.05)。结论雷丸和吡喹酮在体内对猬裂头蚴感染小鼠有一定的治疗作用,雷丸的疗效优于吡喹酮。
Objective To understand the effect of Lei Pill and Praziquantel on the therapeutic efficacy of hedgehog. Method (1) Spider larvae were seized from black spot frog body. One hundred and twelve Kunming mice were divided into 14 groups (A-I). Each mouse was infected with 5 sporozoites orally, 8 mice in each group, and each group was infected with 40 sporozoites. (2) One week after infection, mice in AC group were treated with praziquantel intragastrically for 1 course (once / d × 3 days). The total dose of praziquantel for 1 course was 1 500 mg / kg, 3 000 mg / kg and 6 000 mg / kg, respectively. The DF group was treated with Rayball intragastrically for one course of treatment (once per day for 3 days). The total dose of Rayleigh for 1 course of treatment was 1 200 mg / kg, mg / kg and 4 800 mg / kg. (3) The mice in HJ group and KM group were treated with praziquantel and Lei Pill for one course of treatment at intervals of 7 days for another course of treatment. The total dose of HJ group was the same as that of AC and KM groups ; Group G and Group N mice were instilled with saline only and served as the control group for AF and HM mice respectively. All treatment groups and control group were sacrificed one week after the end of treatment, and the number of seizures and the worm reduction rate of each group were counted. Results (1) Treatment of one course of treatment: There was no significant difference in the worm reduction rate between group A and group B compared with group G (P> 0.05). The average seizure frequency of group C was 2.13 (P <0.05). The worm reduction rates of mice in groups D, E and F were 48.21% and 51.79, respectively % And 44.90%, respectively, compared with the G control group, the difference was statistically significant (P <0.05). (2) The treatment of two courses of treatment: there was no significant difference in the worm reduction rates of the sporozoite between the H, I and J groups and the N control group (P> 0.05) The rates were 48.22%, 55.62% and 59.17%, respectively, compared with the N control group, the difference was statistically significant (P <0.05), there was no significant difference in the worm reduction rates between the treatment groups > 0.05). Conclusion Rayleigh and praziquantel in vivo treatment of hedgehogs infected mice have a certain role in the treatment of mice, Lei Pill better than praziquantel.