论文部分内容阅读
单链抗体 (Sc Fv)是用基因工程方法将抗体重链可变区 (VH)和轻链可变区 (VL)通过一段连接肽 (L inker)连接而成的重组蛋白 ,是保持了亲本抗体的抗原亲和活性和特异性的最小功能性抗体片段 .与完整抗体 Ig G及抗体 Fab段相比 ,具有在非靶向组织中滞留时间短、免疫原性低、血液清除快、肿瘤穿透力强、可在细菌中很经济地生产并可用基因工程的方法构建与其他效应分子连接的抗肿瘤融合蛋白等优点 .因此 Sc Fv成为将药物、毒素、放射性毒素导向肿瘤的很有价值的分子 ,在治疗和诊断试剂的发展上具有巨大的潜力
ScFv is a recombinant protein genetically engineered to link the antibody heavy chain variable region (VH) and light chain variable region (VL) through a linker (Linker) Antibody affinity affinity and specificity of the smallest functional antibody fragment compared with the intact antibody Ig G and antibody Fab segment, with non-target tissue retention time is short, low immunogenicity, rapid clearance of blood, tumor wear Permeable, can be economically produced in bacteria and can be genetically engineered to construct anti-tumor fusion proteins linked to other effector molecules, etc. Thus Sc Fv has been of great value in directing drugs, toxins, and radiotoxins to tumors Molecules, have tremendous potential for the development of therapeutic and diagnostic reagents