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通过盆栽试验,研究了CF(单施化肥)、CM(牛粪)和CM+CF(牛粪和化肥各提供50%的氮)等处理对侧柏根系形态特征、根际土壤微生物数量及酶活性的影响。结果表明:同CF处理相比,CM+CF处理显著提高了侧柏的根表面积、比根表面积、总根长、根尖数和根系活力,其中根表面积分别比CK、CF和CM处理提高110.01%、59.27%和28.54%;明显增加了根际土壤微生物数量及微生物量碳、氮含量,其中微生物量碳、氮含量分别比CF处理增加62.22%和45.35%。CM和CM+CF处理的微生物多样性指数与微生物总量的变化趋势一致,而CK和CF处理的变化趋势相反。此外,CM+CF处理还显著提高了根际土壤脲酶、过氧化氢酶、多酚氧化酶和蔗糖酶活性,尤其对多酚氧化酶的提高幅度最大。与CM+CF处理相比,CM处理对侧柏根际微域的影响较小。因此,牛粪与化肥配施更有利于土壤微生物数量和酶活性的提高,从而为侧柏生长创造良好的微生态环境。
Pot experiments were conducted to study the effects of CF (single chemical fertilizer), CM (cow dung) and CM + CF (50% of nitrogen supplied by cow dung and chemical fertilizers) on root morphological characteristics, rhizosphere soil microbial quantity and enzyme Effect of activity. The results showed that CM + CF treatment significantly increased the root surface area, specific root surface area, total root length, root tip number and root activity of the oriental arborvitae, and the root surface area increased by 110.01 compared with CK, CF and CM treatment, respectively %, 59.27% and 28.54% respectively. The amount of rhizosphere soil microbial biomass carbon and nitrogen were significantly increased. The contents of microbial biomass C and N were increased by 62.22% and 45.35%, respectively. The microbial diversity indices treated with CM and CM + CF were consistent with the trends of the total amount of microorganisms, while the trends of CK and CF treatments were opposite. In addition, CM + CF treatment also significantly increased rhizosphere soil urease, catalase, polyphenol oxidase and invertase activity, especially for polyphenol oxidase increased the most. Compared with CM + CF treatment, the effect of CM treatment on the rhizosphere microdomains was less. Therefore, cow dung and fertilizer application is more conducive to the improvement of soil microbial quantity and enzyme activity, thus creating a good micro-ecological environment for the growth of P. orientalis.