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目的探讨燕麦葡聚糖对高胆固醇血症大鼠血管内皮活性物质及炎症反应标志物C-反应蛋白(CRP)的影响,为防治动脉粥样硬化提供理论依据。方法雄性SD大鼠44只随机分为正常对照组(灌饲生理盐水)11只,高胆固醇模型组(以下简称模型组)33只。模型组给予高脂饲料,正常对照组给予基础饲料,连续饲养4周后测定大鼠空腹血清总胆固醇(TC),以模型组血清TC水平显著高于正常对照组为确定造模成功的指标。将高胆固醇血症大鼠随机分为3组,高胆固醇对照组、葡聚糖高低剂量组[剂量分别为1 000和500 mg/(kg.d)],两个对照组灌胃生理盐水,实验结束,取血测定一氧化氮(NO)、内皮素(ET-1)、血栓素(TXA2)和CRP含量。结果与高胆固醇对照组比较,燕麦葡聚糖能够升高NO水平,降低ET-1、TXA2含量,且一定剂量的燕麦葡聚糖可以降低CRP水平。结论燕麦葡聚糖具有一定的改善血管内皮舒缩功能,抑制血小板聚集,减轻内皮炎症反应,具有保护血管内皮的作用。
Objective To investigate the effect of oat glucan on vascular endothelium and the level of C-reactive protein (CRP) in hypercholesterolemic rats and to provide a theoretical basis for the prevention and treatment of atherosclerosis. Methods Forty-four male Sprague-Dawley rats were randomly divided into normal control group (fed with normal saline) 11 and high cholesterol model group (hereinafter referred to as model group). The model group was given high-fat diet, and the normal control group was given basic diet. The fasting serum total cholesterol (TC) was determined 4 weeks after continuous feeding. The serum TC level in the model group was significantly higher than that in the normal control group. The hypercholesterolemic rats were randomly divided into three groups: high cholesterol control group, dextran high and low dose groups (doses of 1000 and 500 mg / (kg.d)], respectively. The two control groups were given normal saline, At the end of the experiment, the levels of nitric oxide (NO), endothelin (ET-1), thromboxane (TXA2) and CRP were determined by blood sampling. Results Compared with the high cholesterol control group, oat dextran increased NO levels and ET-1 and TXA2 levels, and a certain dose of oat glucan could reduce CRP levels. Conclusion Oatmeal has a certain role in improving vascular endothelium, reducing platelet aggregation, reducing endothelium inflammatory reaction and protecting vascular endothelium.