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钝顶螺旋藻（Sp.－D）藻胆蛋白的粗提比较方便，用0．001mol／L磷酸盐缓冲液浸提，经反复冻融（三次）处理，可以获得纯度（AλAmax／A280）为0．54的藻胆蛋白粗提液；对粗提液用硫酸铵盐析，可使其纯度略有提高，达到0．59左右，但粗提液中藻蓝蛋白（PC）和别藻蓝蛋白（APC）仍未分离开：进一步通过连续两次羟基磷灰石（HA）柱层析纯化之后，藻蓝蛋白（PC）的纯度可达到4.1，别藻蓝蛋白（APC）的纯度可达到3．5。
The crude extract of Sp.-D phycobiliprotein was more convenient and was extracted with0.001mol / L phosphate buffer. After repeated freeze-thawing (three times), the purity (AλAmax / A280) was 0.54 of phycobiliprotein crude extract; crude extract with ammonium sulfate salting-out, its purity can be slightly increased, reaching about 0.59, but the crude extract of phycocyanin (PC) and algae blue Protein (APC) still has not been separated: After further purification by two consecutive hydroxyapatite (HA) column chromatographies, the purity of phycocyanin (PC) reaches 4.1 and the purity of allophycocyanin (APC) 3.5.