【摘 要】
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Peripheral nerve injury could lead to chronic neuropathic pain.Understanding transcriptional changes induced by nerve injury could provide fundamental insights into the complex pathogenesis of neuropathic pain.Gene expression profiles of dorsal root gangl
【机 构】
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Institute of Neuroscience,State Key Laboratory of Neuroscience,CAS Center for Excellence in Brain Sc
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Peripheral nerve injury could lead to chronic neuropathic pain.Understanding transcriptional changes induced by nerve injury could provide fundamental insights into the complex pathogenesis of neuropathic pain.Gene expression profiles of dorsal root ganglia(DRG)in neuropathic pain condition have been studied.However,little is known about transcriptomic changes in individual DRG neurons after peripheral nerve injury.Here we performed single-cell RNA sequencing on dissociated mouse DRG cells after spared nerve injury(SNI).In addition to DRG neuron types.that are found under physiological conditions,we identified three SNI-induced neuronal clusters(SNIICs)characterized by the expression of Atf3/Gfra3/Gal(SNIIC1),Atf3/Mrgprd(SNIIC2)and Atf3/S100b/Gal(SNIIC3).These SNIICs originated from Cldn9+/Gal+,Mrgprd+ and Trappc3l+ DRG neurons,respectively.Interestingly,SNIIC2 switched to SNIIC1 by increasing Gal and reducing Mrgprd expression 2 days after nerve injury.Inferring the gene regulatory networks after nerve injury,we revealed that activated transcription factors Atf3 and Egr1 in SNIICs could enhance Gal expression while activated Cpeb1 in SNIIC2 might suppress Mrgprd expression within 2 days after SNI.Furthermore,we mined the transcriptomic changes in the development of neuropathic pain to identify potential analgesic targets.We revealed that cardiotrophin-like cytokine factor 1,which activates astrocytes in the dorsal horn of spinal cord,was upregulated in SNIIC1 neurons and contributed to SNI-induced mechanical allodynia.Therefore,our results provide a new landscape to understand the dynamic course of neuron type changes and their underlying molecular mechanisms during the development of neuropathic pain.
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