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αB-晶体蛋白(αB-crystallin)是小热休克蛋白(Small heat shock protein,sHSP)家族的代表成员,已发现与多种神经退行性疾病发生发展密切相关,但至今为止对αB-晶体蛋白在朊病毒病发生发展中可能扮演的角色研究甚少,作用尚不清楚。本研究利用羊瘙痒因子仓鼠敏感株263K感染仓鼠建立的朊病毒实验动物模型,通过免疫印迹(Western blots)和免疫组织化学染色观察到伴随PrPSc在感染终末期动物脑组织中大量沉积,αB-晶体蛋白表达显著上调,比正常对照增高3倍。免疫荧光双染确定其分布的主要细胞类型为星形胶质细胞,神经元中未检测到表达。感染动物中高表达的αB-晶体蛋白与异常沉积的PrPSc无明显共定位。此研究为进一步探讨和揭示αB-晶体蛋白在朊病毒病中可能的作用和分子机制奠定了基础。
αB-crystallin is a representative member of the small heat shock protein (sHSP) family. It has been found that αB-crystallin is closely related to the development and progression of a variety of neurodegenerative diseases. However, αB- The possible role of prion disease in the development and progression of the disease is poorly understood and its role is unclear. In the present study, a prion experimental animal model of hamster infecting hamsters with the pruritus-susceptible hamster strain 263K was used. PrPECs were abundantly precipitated in the brain tissue of infected end-stage animals by Western blot and immunohistochemical staining. Protein expression was significantly up-regulated by 3-fold over the normal control. Immunofluorescence double staining to determine the distribution of the main cell type astrocytes, neurons were not detected in the expression. The highly expressed αB-crystallin in infected animals showed no significant colocalization with abnormally deposited PrPSc. This study lays a foundation for further exploration and revealing the possible role and molecular mechanism of αB-crystallin in prion diseases.