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为了探讨基于DNA分子标记构建平菇栽培品种核心样本的方法,基于48个平菇栽培菌株的11个SSR位点,根据遗传距离进行UPGMA聚类,采用位点优先取样策略,结合表型性状,在不同SSR等位基因保留比例(100%、95%、90%、85%、80%)水平上构建核心样本,进而确定取样量。采用稀有等位基因保留比例以及对Nei’s基因多样度和Shannon’s信息指数进行t检验,评价核心样本的代表性,并且根据表型保留比例以及极差、均值和标准差的符合率对核心样本的代表性作进一步确认。结果表明:采用位点优先取样策略,在等位基因保留比例为95%的水平上构建的核心样本能够以最小的样本量最大限度地代表原种质的遗传多样性。
In order to explore a method for constructing core samples of Pleurotus ostreatus based on DNA molecular markers, based on 11 SSR loci of 48 Pleurotus ostreatus cultivars, UPGMA clustering was carried out according to genetic distance. Site-prioritized sampling strategy was used to combine the phenotypic traits, Core samples were constructed at different SSR allele retention ratios (100%, 95%, 90%, 85%, 80%) to determine the sample size. Representatives of core samples were evaluated using rare allele retention ratios and t-tests for Nei’s gene diversity and Shannon’s information index, and representative of the core sample based on the proportion of phenotype retention and the coincidence of mean, standard deviation and standard deviation Sex for further confirmation. The results showed that using the site-first sampling strategy, the core samples constructed at the level of 95% of allele retention could represent the genetic diversity of the original germplasm to the maximum with the smallest sample size.